PIP4K2A Antibody - N-terminal region (OAGA02733)

Catalog No: OAGA02733

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: H1299
10% SDS PAGE
OAGA02733 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded tyroid ca, using PIP5K2 alpha (OAGA02733) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using PIP5K2 alpha (OAGA02733) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IF, IHC-P, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PIP5K2 alpha. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Target Info

• Gene Symbol: PIP4K2A
• Gene Full Name: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• Alias Symbols: PIPK, PI5P4KA, PIP5K2A, PIP5KIIA, PIP5KII-alpha
• NCBI Gene Id: 5305
• Description of Target: Phosphatidylinositol-5,4-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-5,4-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family.
• Uniprot ID: P48426