Catalog No: OKCD02771
Size:96WELLS
Price: $800.00
SKU
OKCD02771
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for PIP ELISA Kit (Rat) (OKCD02771)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%78-93%81-101%85-92%
EDTA Plasma (n=5)98-105%78-91%80-99%80-95%
Heparin Plasma (n=5)83-93%96-104%82-104%83-94%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolactin Induced Protein (PIP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolactin Induced Protein (PIP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolactin Induced Protein (PIP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prolactin Induced Protein (PIP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range46.88-3000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)87-9592
EDTA Plasma (n=5)95-10299
Heparin Plasma(n=5)85-9589
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolactin Induced Protein (PIP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolactin Induced Protein (PIP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PIP Microplate96 Wells (12 x 8 Well strips)
PIP Lyophilized Standard2
100X Biotinylated PIP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity16.23 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Prolactin Induced Protein (PIP). No significant cross-reactivity or interference between Prolactin Induced Protein (PIP) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolPip
Gene Full Nameprolactin induced protein
Alias SymbolsProlactin-induced protein;prolactin-inducible protein homolog.
NCBI Gene Id64673
Protein NameProlactin-inducible protein homolog
Description of TargetProlactin-inducible protein homolog
Uniprot IDO70417
Protein Accession #NP_073199.1
Nucleotide Accession #NM_022708.2
Protein Size (# AA)146
Write Your Own Review
You're reviewing:PIP ELISA Kit (Rat) (OKCD02771)
Your Rating
We found other products you might like!