PIP ELISA Kit (Bovine) (OKEH03940)

Catalog No: OKEH03940

Size:96T

Bovine PIP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PIP ELISA Kit (Bovine) (OKEH03940) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human PIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PIP and incubated. The PIP found in the sample or standards competes with the biotinylated PIP for limited binding sites on the immobilized anti-human PIP antibody. Excess unbound biotinylated PIP and sample or standard PIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PIP captured in the well and inversely proportional to the amount of PIP which was contained in the sample or standard.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Component:
  • Anti-PIP Microplate: 96 Wells (12 x 8 Well strips)
  • PIP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PIP-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.17 ng/mL
• Specificity: Natural and recombinant Bovine Prolactin-inducible protein homolog
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PIP
• Gene Full Name: prolactin induced protein
• Alias Symbols: prolactin inducible protein;Prolactin-induced protein;prolactin-inducible protein homolog.
• NCBI Gene Id: 613853
• Protein Name: P60986
• Description of Target: Prolactin-inducible protein homolog
• Uniprot ID: https://www.uniprot.org/uniprot/P60986
• Protein Accession #: NP_001074382.1
• Nucleotide Accession #: NM_001080913.1
• Protein Size (# AA): 145
• Molecular Weight: 145 kDa