Catalog No: OKEH03672
Size:96 Wells
Price: $600.00
SKU
OKEH03672
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PINP ELISA Kit (Mouse) (OKEH03672) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PINP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for PINP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PINP captured in well.
ELISA Kit Range78 - 5000pg/mL
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.6% (n = 20)
Mean Inter-assay CV%: < 10.2% (n = 20)
ELISA Kit Component
ComponentAmount
PINP Microplate96 Wells (12 x 8 Well strips)
PINP Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PINP Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity39.7 pg/mL
SpecificityNatural and recombinant Mouse N-terminal propeptide of Collagen alpha-1(I) chain
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPINP
Gene Full NameN-terminal propeptide of Collagen alpha-1(I) chain
Alias SymbolsN-terminal propeptide of Collagen alpha-1(I) chain
Protein NameN-terminal propeptide of Collagen alpha-1(I) chain
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