PINK1 Antibody - N-terminal region (OAAB16695)

Catalog No: OAAB16695

Size:400ul

Anti-Park6 (PINK1) Antibody (N-term) at 1:1000 dilution + A549 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 63 kDa

Formalin-fixed and paraffin-embedded human human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid.PBS with 0.09% (W/V) sodium azide
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Cellular Location: Mitochondrion outer membrane; Single-pass membrane protein. Mitochondrion inner membrane; Single-pass membrane protein. Cytoplasm, cytosol. Note=Localizes mostly in mitochondrion and the 2 proteolytic processed fragments of 55 kDa and 48 kDa localize mainly in cytosol
  Tissue Location: Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PINK1 (PARK6) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-147 amino acids from the N-terminal region of human PINK1 (PARK6).
• Purification: This antibody is purified by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Sample Type Confirmation:

  PINK1 is supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1,000
  IHC-P: 1:50~100
  Optimal dilutions should be determined by the end user.
• Reference: Rogaeva,E.,et al. Arch. Neurol. 61 (12), 1898-1904 (2004)
  Hatano,Y., et al. Ann. Neurol. 56 (3), 424-427 (2004)
  Healy,D.G., et al. Ann. Neurol. 56 (3), 329-335 (2004)
  Valente,E.M., et al. Science 304 (5674), 1158-1160 (2004)
  Nakajima,A., et al. Cancer Lett. 201 (2), 195-201 (2003)
  Unoki,M. and Nakamura,Y. Oncogene 20 (33), 4457-4465 (2001)

Target Info

• Gene Symbol: PINK1
• Alias Symbols: BRPK, PARK6
• NCBI Gene Id: 65018
• Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• Description of Target: Defects in PINK1 are the cause of autosomal recessive early-onset Parkinson's disease 6 (PARK6). Six novel pathogenic PINK1 mutations suggest that PINK1 may be the second most common causative gene next to parkin in parkinsonism with the recessive mode of inheritance. Strong evidence indicates that, although important in mendelian forms of Parkinson's disease (PD), PINK1 does not influence the cause of sporadic nonmendelian forms of PD.
• Uniprot ID: Q9BXM7
• Protein Accession #: NP_115785.1
• Molecular Weight: 63 kDa