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Catalog No: OKCD01170 (Formerly GWB-KBBDJ7)
Size:96WELLS
Price: $750.00
SKU
OKCD01170
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Datasheets/ManualsPrintable datasheet for PIM2 ELISA Kit (Human) (OKCD01170)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)96-103%92-105%95-105%79-89%
EDTA plasma(n=5)78-96%80-96%85-96%91-99%
heparin plasma(n=5)81-104%78-92%88-96%89-105%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pim-2 Oncogene (PIM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pim-2 Oncogene (PIM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pim-2 Oncogene (PIM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pim-2 Oncogene (PIM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)91-9995
EDTA plasma(n=5)96-105101
heparin plasma(n=5)94-10297
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pim-2 Oncogene (PIM2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pim-2 Oncogene (PIM2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PIM2 Microplate96 Wells (12 x 8 Well Strips)
PIM2 Lyophilized Standard2 x 40 ng
100X Biotinylated PIM2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.257 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pim-2 Oncogene (PIM2). No significant cross-reactivity or interference between Pim-2 Oncogene (PIM2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPIM2
Gene Full NamePim-2 proto-oncogene, serine/threonine kinase
Alias Symbolspim-2 oncogene;pim-2h;proto-oncogene Pim-2 (serine threonine kinase);serine/threonine-protein kinase pim-2.
NCBI Gene Id11040
Protein NameSerine/threonine-protein kinase pim-2
Description of TargetProto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Uniprot IDQ9P1W9
Protein Accession #NP_006866
Nucleotide Accession #NM_006875.3
Protein Size (# AA)311
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