PIKFYVE Antibody (Phospho-Ser307) (OASG05879)

Catalog No: OASG05879

Size:100 uL

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC, IF
• Reconstitution and Storage: Store at -20C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human PIP5KIII around the phosphorylation site of S307. Location: 250-330aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Ser307
• Specificity: Phospho-PIP5KIII (S307) Polyclonal Antibody detects endogenous levels of PIP5KIII protein only when phosphorylated at S307.
• Application Info: IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000;

Target Info

• Gene Symbol: PIKFYVE
• Gene Full Name: phosphoinositide kinase, FYVE finger containing
• Alias Symbols: CFD, FAB1, HEL37, PIP5K, PIP5K3, ZFYVE29
• NCBI Gene Id: 200576; 18711;
• Description of Target: Phosphorylated derivatives of phosphatidylinositol (PtdIns) regulate cytoskeletal functions, membrane trafficking, and receptor signaling by recruiting protein complexes to cell- and endosomal-membranes. Humans have multiple PtdIns proteins that differ by the degree and position of phosphorylation of the inositol ring. This gene encodes an enzyme (PIKfyve; also known as phosphatidylinositol-3-phosphate 5-kinase type III or PIPKIII) that phosphorylates the D-5 position in PtdIns and phosphatidylinositol-3-phosphate (PtdIns3P) to make PtdIns5P and PtdIns(3,5)biphosphate. The D-5 position also can be phosphorylated by type I PtdIns4P-5-kinases (PIP5Ks) that are encoded by distinct genes and preferentially phosphorylate D-4 phosphorylated PtdIns. In contrast, PIKfyve preferentially phosphorylates D-3 phosphorylated PtdIns. In addition to being a lipid kinase, PIKfyve also has protein kinase activity. PIKfyve regulates endomembrane homeostasis and plays a role in the biogenesis of endosome carrier vesicles from early endosomes. Mutations in this gene cause corneal fleck dystrophy (CFD); an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. Histologically, these flecks appear to be keratocytes distended with lipid and mucopolysaccharide filled intracytoplasmic vacuoles. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Uniprot ID: Q9Y2I7, Q9Z1T6
• Molecular Weight: 237 kDa