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Catalog No: OKEH02353
Price: $625.00
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PIK3CD ELISA Kit (Human) : 96 Wells (OKEH02353)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PIK3CD ELISA Kit (Human) (OKEH02353) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CD captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
PIK3CD Microplate96 Wells (12 x 8 Well strips)
PIK3CD Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated PIK3CA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.089 ng/mL
SpecificityNatural and recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPIK3CD
Gene Full Namephosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta
Alias SymbolsAPDS;IMD14;IMD14A;IMD14B;p110D;P110DELTA;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 20;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8/20;phosphoinositide-3-kinase C;phosphoinositide-3-kinase, catalytic, delta polypeptide variant p37delta;PI3K;PI3Kdelta;PI3-kinase p110 subunit delta;ptdIns-3-kinase subunit p110-delta;ROCHIS.
NCBI Gene Id5293
Protein NameO00329
Description of TargetPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform
Uniprot ID
Protein Accession #NP_005017.3
Nucleotide Accession #NM_005026.3
Protein Size (# AA)1044
Molecular Weight119 kDa
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