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Catalog No: OKDD00466 (Formerly GWB-KBBCY7)
Size:96 Wells
Price: $800.00
SKU
OKDD00466
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Datasheets/ManualsPrintable datasheet for PIK3Cb ELISA Kit (Human) (OKDD00466)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked immunosorbent assay
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology PIK3Cb ELISA Kit (Human) (OKDD00466) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for PIK3Cb has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3Cb is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PIK3Cb captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PIK3CB were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PIK3CB were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

ELISA Kit Component
ComponentAmount
Anti-PIK3Cb Microplate96 Wells (12 x 8 Well Strips)
PIK3Cb Lyophlized Standard2 x 10 ng
Standard Diluent1 x 20 mL
100X PIK3Cb Biotinylated Detector Antibody1 x 120 uL
100X Avidin-HRP Detection Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Detection Conjugate Diluent1 x 12 mL
10X Sample Diluent1 x 30 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of PIK3CB. No significant cross-reactivity or interference between PIK3CB and analogues was observed.
Assay InfoQuantitative Sandwich ELISA
Gene SymbolPIK3CB
Gene Full Namephosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
Alias SymbolsP110BETA;phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit beta;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform;phosphoinositide-3-kinase, catalytic, beta polypeptide;PI3K;PI3K-beta;PI3KBETA;PI3-kinase p110 subunit beta;PI3-kinase subunit beta;PIK3C1;PtdIns-3-kinase p110;ptdIns-3-kinase subunit beta;ptdIns-3-kinase subunit p110-beta.
NCBI Gene Id5291
Protein NamePhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform
Description of TargetPhosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.
Uniprot IDP42338
Protein Accession #NP_006210.1
Nucleotide Accession #NM_006219.2
Protein Size (# AA)1070
Molecular Weight123 kDa
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