PIK3CB Antibody (OAAN03186)

Catalog No: OAAN03186

Western blot analysis of extracts of various cell lines, using PIK3CB antibody.

Immunohistochemistry of paraffin-embedded mouse lung using PIK3CB antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung using PIK3CB antibody at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon carcinoma using PIK3CB antibody at dilution of 1:100 (40x lens).

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: WB, IHC
• Reconstitution and Storage: Store at +4C. For long term storage, aliquote and store at -20C. Avoid freeze/thaw cycles.
• Immunogen: Recombinant Protein
• Purification: Affinity purification
• Preservative: 0.02% Sodium Azide
• Formulation: PBS with 0.02% sodium azide, 50% glyceron, pH 7.3
• Application Info: WB 1:500 - 1:2,000
  IHC 1:50 - 1:100

Target Info

• Gene Symbol: PIK3CB
• Gene Full Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit beta
• Alias Symbols: PI3K, PIK3C1, P110BETA, PI3KBETA
• NCBI Gene Id: 5291
• Protein Name: Ras-related protein Rap-1A
• Description of Target: Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Involved in the activation of AKT1 upon stimulation by G-protein coupled receptors (GPCRs) ligands such as CXCL12, sphingosine 1-phosphate, and lysophosphatidic acid. May also act downstream receptor tyrosine kinases. Required in different signaling pathways for stable platelet adhesion and aggregation. Plays a role in platelet activation signaling triggered by GPCRs, alpha-IIb/beta-3 integrins (ITGA2B/ ITGB3) and ITAM (immunoreceptor tyrosine-based activation motif)-bearing receptors such as GP6. Regulates the strength of adhesion of ITGA2B/ ITGB3 activated receptors necessary for the cellular transmission of contractile forces. Required for platelet aggregation induced by F2 (thrombin) and thromboxane A2 (TXA2). Has a role in cell survival. May have a role in cell migration. Involved in the early stage of autophagosome formation. Modulates the intracellular level of PtdIns3P (Phosphatidylinositol 3-phosphate) and activates PIK3C3 kinase activity. May act as a scaffold, independently of its lipid kinase activity to positively regulate autophagy. May have a role in insulin signaling as scaffolding protein in which the lipid kinase activity is not required. May have a kinase-independent function in regulating cell proliferation and in clathrin-mediated endocytosis. Mediator of oncogenic signal in cell lines lacking PTEN. The lipid kinase activity is necessary for its role in oncogenic transformation. Required for the growth of ERBB2 and RAS driven tumors.
• Uniprot ID: P42338
• Protein Accession #: NP_006210.1
• Nucleotide Accession #: NM_006219.2
• Molecular Weight: 123 kDa