PIK3CA ELISA Kit (Human) : 96 Wells (OKEH02351)

Catalog No: OKEH02351 (Formerly GWB-40FTIU)

Size:96T

Human PIK3CA ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: ELISA-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PIK3CA ELISA Kit (Human) (OKEH02351) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CA captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.8% (n = 20)
  Mean Inter-assay CV%: < 9.1% (n = 20)
• ELISA Kit Component:
  • PIK3CA Microplate: 96 Wells (12 x 8 Well strips)
  • PIK3CA Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated YWHZ Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.056 ng/mL
• Specificity: Natural and recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PIK3CA
• Gene Full Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
• Alias Symbols: CLAPO;CLOVE;CWS5;MCAP;MCM;MCMTC;mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha;p110-alpha;phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha;phosphatidylinositol 3-kinase, catalytic, alpha polypeptide;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;phosphoinositide 3-kinase alpha;Phosphoinositide-3-kinase catalytic alpha polypeptide;phosphoinositide-3-kinase, catalytic, alpha polypeptide;PI3K;PI3K-alpha;PI3-kinase p110 subunit alpha;ptdIns-3-kinase subunit p110-alpha;serine/threonine protein kinase PIK3CA.
• NCBI Gene Id: 5290
• Protein Name: P42336
• Description of Target: Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
• Uniprot ID: https://www.uniprot.org/uniprot/P42336
• Protein Accession #: NP_006209.2
• Nucleotide Accession #: NM_006218.2
• Protein Size (# AA): 1068
• Molecular Weight: 124 kDa