Catalog No: OKEH02351 (Formerly GWB-40FTIU)
Price: $625.00
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PIK3CA ELISA Kit (Human) : 96 Wells (OKEH02351)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PIK3CA ELISA Kit (Human) (OKEH02351) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CA captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 90%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 9.1% (n = 20)
ELISA Kit Component
PIK3CA Microplate96 Wells (12 x 8 Well strips)
PIK3CA Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated YWHZ Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.056 ng/mL
SpecificityNatural and recombinant Human Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPIK3CA
Gene Full Namephosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha
Alias SymbolsCLAPO;CLOVE;CWS5;MCAP;MCM;MCMTC;mutant phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha;p110-alpha;phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha;phosphatidylinositol 3-kinase, catalytic, alpha polypeptide;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;phosphoinositide 3-kinase alpha;Phosphoinositide-3-kinase catalytic alpha polypeptide;phosphoinositide-3-kinase, catalytic, alpha polypeptide;PI3K;PI3K-alpha;PI3-kinase p110 subunit alpha;ptdIns-3-kinase subunit p110-alpha;serine/threonine protein kinase PIK3CA.
NCBI Gene Id5290
Protein NameP42336
Description of TargetPhosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform
Uniprot ID
Protein Accession #NP_006209.2
Nucleotide Accession #NM_006218.2
Protein Size (# AA)1068
Molecular Weight124 kDa
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