PIK3C3 ELISA Kit (Human) (OKCD00715)

Catalog No: OKCD00715 (Formerly GWB-KBBYZ5)

Size:96WELLS

Human PIK3C3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  	78-90%	91-102%	81-102%	85-94%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class 3 (PIK3C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class 3 (PIK3C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphoinositide-3-Kinase Class 3 (PIK3C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  	82-95	90

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Class 3 (PIK3C3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphoinositide-3-Kinase Class 3 (PIK3C3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PIK3C3 Microplate: 96 Wells (12 x 8 Well Strips)
  • PIK3C3 Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated PIK3C3 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.59 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Phosphoinositide-3-Kinase Class 3 (PIK3C3). No significant cross-reactivity or interference between Phosphoinositide-3-Kinase Class 3 (PIK3C3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PIK3C3
• Gene Full Name: phosphatidylinositol 3-kinase catalytic subunit type 3
• Alias Symbols: hVps34;phosphatidylinositol 3-kinase catalytic subunit type 3;phosphatidylinositol 3-kinase p100 subunit;Phosphoinositide-3-kinase class 3;phosphoinositide-3-kinase, class 3;PI3K type 3;PI3-kinase type 3;PtdIns-3-kinase type 3;VPS34.
• NCBI Gene Id: 5289
• Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• Description of Target: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).
• Uniprot ID: Q8NEB9
• Protein Accession #: NP_002638
• Nucleotide Accession #: NM_002647.3
• Protein Size (# AA): 887