PIG MACROPHAGES Antibody (OASA11733)

Catalog No: OASA11733

Size:0.1mg

Product Info

• Predicted Species Reactivity: Pig
• Product Format: Purified IgG - liquid
• Clonality: Monoclonal
• Clone: BA4D5
• Isotype: IgG2b
• Host: Mouse
• Application: IHC-AFF, FC, IP, WB
• Additional Information: Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 mouse myeloma cell line.
  Preservative Stabilisers: 0.09% Sodium Azide (NaN3)
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solution: Phosphate buffered saline
• Reconstitution and Storage: Store at +4C or at -20C if preferred.
  Storage in frost-free freezers is not recommended.
  This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
  
• Immunogen: Porcine alveolar macrophages.
• Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Predicted Homology Based on Immunogen Sequence: Pig
• Specificity: Clone BA4D5 recognises porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.
  Some expression has also been observed on peripheral blood lymphocytes.
  The antigen recognised by clone BA4D5 has a broad tissue distribution and this antibody stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches. Expression has also been reported on some non-heamatopoietic cells including endothelial cells.
  It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognised by this antibody has not yet been fully characterised. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
  
• Application Info: Flow Cytometry: Suggested dilution: 1/50-1/100
  Membrane permeabilization is required for this application. Aviva recommends the use of Leucoperm for this purpose. Use 10ul of the suggested working dilution to label 1x10⁶cells in 100ul.
  Western Blotting: Clone BA4D5 recognizes a 105kDa antigen in pig macrophage lysates under non-reducing conditions.