PIEZO1 ELISA Kit (Human) (OKEH03533)

Catalog No: OKEH03533

Size:96T

Human PIEZO1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PIEZO1 ELISA Kit (OKEH03533) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-PIEZO1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated PIEZO1 and incubated. The PIEZO1 found in the sample or standards competes with the biotinylated PIEZO1 for limited binding sites on the immobilized anti-PIEZO1 antibody. Excess unbound biotinylated PIEZO1 and sample or standard PIEZO1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated PIEZO1 captured in the well and inversely proportional to the amount of PIEZO1 which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 103%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.0% (n = 20)
  Mean Inter-assay CV%: < 6.3% (n = 20)
• ELISA Kit Component:
  • PIEZO1 Microplate: 96 Wells (12 x 8 Well strips)
  • PIEZO1 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X PIEZO1-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Piezo-type mechanosensitive ion channel component 1
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: PIEZO1
• Gene Full Name: piezo type mechanosensitive ion channel component 1
• Alias Symbols: DHS;FAM38A;family with sequence similarity 38, member A;LMPH3;LMPHM6;membrane protein induced by beta-amyloid treatment;Mib;piezo-type mechanosensitive ion channel component 1;Protein FAM38A.
• NCBI Gene Id: 9780
• Protein Name: Q92508
• Description of Target: Piezo-type mechanosensitive ion channel component 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q92508
• Protein Accession #: NP_001136336.2
• Nucleotide Accession #: NM_001142864.3
• Protein Size (# AA): 2521
• Molecular Weight: 287 kDa