Piccolo Antibody : FITC (OASE00136-FITC)

Catalog No: OASE00136-FITC

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 6H9-B6
• Isotype: IgG2b Kappa
• Host: Mouse
• Conjugation: FITC: Fluorescein Isothiocyanate
• Application: WB, ELISA
• Additional Information: Background Info: No cross-reactivity to Bassoon, band at ~550kDa corresponding to the molecular weight of Piccolo.
  Scientific Background: Piccolo, also referred to as Aczonin, is a large protein which consists of an N-terminal Zn2+ finger, several
  piccolo-bassoon homology domains and C-terminal PDZ
  and C2 domains (1, 2). In general it is found together
  with bassoon, a related huge multi-domain protein of the
  CAZ (cytoskeletal matric assembled at active zones) (3).
  Piccolo is a scaffolding protein for proteins involved in
  endo- and exocytosis of synaptic vesicles. Piccolo has
  also been shown to interfere with clathrin medicated
  endocytosis by binding to the F-actin and dynamin
  binding protein Abp1 (2).
  Certificate of Analysis: 1ug/mL was sufficient for detection of Piccolo in 20ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Full length protein, BC001304.1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Gerber S.H., Garcia J., Rizo J., Sudhof T.C. (2001) EMBO J. 20(7): 1605-1619.
  2. Wang X., et al. (1999) J Cell Biol. 147(1): 151-162.
  3. Dresbach T., et al. (2006) J Biol Chem. 281(9):6038-
  6047.

Target Info

• Gene Symbol: Pclo
• Gene Full Name: piccolo (presynaptic cytomatrix protein)
• Alias Symbols: P, Ac, Acz, Pico
• NCBI Gene Id: 26875
• Uniprot ID: Q9QYX7
• Protein Accession #: NP_001104266.1