PIAS3 ELISA Kit (Human) (OKCD02800)

Catalog No: OKCD02800

Size:96WELLS

Human PIAS3 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  	80-104%	97-105%	92-101%	80-105%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Inhibitor Of Activated STAT 3 (PIAS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Inhibitor Of Activated STAT 3 (PIAS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Protein Inhibitor Of Activated STAT 3 (PIAS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  	90-101	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Inhibitor Of Activated STAT 3 (PIAS3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Inhibitor Of Activated STAT 3 (PIAS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-PIAS3 Microplate: 96 Wells (12 x 8 Well strips)
  • PIAS3 Lyophilized Standard: 2
  • 100X Biotinylated PIAS3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Inhibitor Of Activated STAT 3 (PIAS3). No significant cross-reactivity or interference between Protein Inhibitor Of Activated STAT 3 (PIAS3) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PIAS3
• Gene Full Name: protein inhibitor of activated STAT 3
• Alias Symbols: E3 SUMO-protein ligase PIAS3;E3 SUMO-protein transferase PIAS3;protein inhibitor of activated STAT protein 3;zinc finger, MIZ-type containing 5;ZMIZ5.
• NCBI Gene Id: 10401
• Protein Name: E3 SUMO-protein ligase PIAS3
• Description of Target: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376).
• Uniprot ID: Q9Y6X2
• Protein Accession #: NP_006090.2
• Nucleotide Accession #: NM_006099.3
• Protein Size (# AA): 628