PIAS2 Antibody (OAEB00930)

Catalog No: OAEB00930 (Formerly GWB-63D46E)

Size:100UG

OAEB00930 (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Info

• Predicted Species Reactivity: Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Clonality: Polyclonal
• Host: Goat
• Application: ELISA|IHC
• Reconstitution and Storage: -20°C
• Immunogen: PIAS2
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 86%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 86%; Rabbit: 100%; Rat: 86%
• Peptide Sequence: KEAMKVSSQPCTKIE
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: antibody detection limit dilution 1:8000.
  Western Blot: Preliminary experiments gave an approx 90kDa band in lysates of cell line A431after 0.5ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 68.2kDa according to NP_004662.2. The 90kDa band was successfully blocked by incubation with the immunizing peptide.

Target Info

• Reference: * van den Akker E, Ano S, Shih HM, Wang LC, Pironin M, Palvimo JJ, Kotaja N, Kirsh O, Dejean A, Ghysdael J.; FLI-1 functionally interacts with PIASxalpha, a member of the PIAS E3 SUMO ligase family.; J Biol Chem. 2005 Nov 11;280(45):38035-46. Epub 2005 Sep 7.; Pubmed 16148010
• Gene Symbol: Pias2|PIAS2
• Gene Full Name: protein inhibitor of activated STAT 2
• Alias Symbols: AI462206, androgen receptor-interacting protein 3, ARIP3, AU018068, DAB2-interacting protein, Di, Dib, DIP, E3 SUMO-protein ligase PIAS2, E3 SUMO-protein transferase PIAS2, Miz, MIZ1, msx-interacting zinc finger protein, Msx-interacting-zinc finger, PI, PIAS, PIASX, PIASxalpha, PIASxalpha6, PIASxb, PIASxbeta, protein inhibitor of activated STAT X, RING-type E3 ubiquitin transferase PIAS2, SIZ2, zinc finger, MIZ-type containing 4, ZMIZ4.
• NCBI Gene Id: 17344|9063
• Protein Name: E3 SUMO-protein ligase PIAS2 isoform alpha [Homo sapiens]|E3 SUMO-protein ligase PIAS2 isoform beta [Homo sapiens]
• Uniprot ID: O75928
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_004662.2,NP_775298.1
• Molecular Weight: 63.4; 68.2