PIAS1 ELISA Kit (Mouse) (OKWB00255)

Catalog No: OKWB00255

Size:96T

Mouse PIAS1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	88-105%	87-105%	89-103%	85-101%
  EDTA Plasma (n=5)	82-99%	88-99%	83-99%	85-99%
  Heparin Plasma (n=5)	83-100%	80-99%	81-92%	82-88%

• ELISA Kit Principle: Aviva Systems Biology Pias1 ELISA Kit (Mouse) (OKWB00255) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pias1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pias1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pias1 captured in the well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	85-102	95
  EDTA Plasma (n=5)	89-99	94
  Heparin Plasma (n=5)	92-102	99

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Pias1 Microplate: 96 Wells (12 x 8 Well strips)
  • Pias1 Lyophilized Standard: 2 x 10 ng
  • 100X Pias1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.094 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Pias1
• Gene Full Name: protein inhibitor of activated STAT 1
• Alias Symbols: 2900068C24Rik;Ddxbp;Ddxbp1;DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1;DEAD/H box-binding protein 1;E3 SUMO-protein ligase PIAS1;GB;GBP;protein inhibitor of activated STAT protein 1;RING-type E3 ubiquitin transferase PIAS1.
• NCBI Gene Id: 56469
• Protein Name: E3 SUMO-protein ligase PIAS1
• Description of Target: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA (By similarity). The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (PubMed:24061474).
• Uniprot ID: O88907
• Protein Accession #: NP_062637
• Nucleotide Accession #: NM_019663