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Datasheets/Manuals | Printable datasheet for PIAS1 Antibody (OAAF01956) |
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Predicted Species Reactivity | Human|Mouse |
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Product Format | Liquid |
Clonality | Polyclonal |
Host | Rabbit |
Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot |
Reconstitution and Storage | -20°C |
Immunogen | The antiserum was produced against synthesized peptide derived from human PIAS1. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
Specificity | PIAS1 antibody detects endogenous levels of total PIAS1 protein. |
Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Dilution | The optimal working dilution should be determined by the end user. |
Application Info | WB: 1:500~1:3000; IHC: 1:50~1:100; ELISA: 1:10000 |
Reference | Bin Liu, Mol. Cell. Biol., Feb 2005; 25: 1113 - 1123. Hansol Lee, Genes & Dev., Apr 2006; 20: 784 - 794. Tamar Megidish, J. Biol. Chem., Mar 2002; 277: 8255 - 8259. Keiko Kawai-Kowase, Mol. Cell. Biol., Sep 2005; 25: 8009 - 8023. |
Gene Symbol | PIAS1 |
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Gene Full Name | protein inhibitor of activated STAT 1 |
Alias Symbols | AR interacting protein;DDXBP1;DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1;DEAD/H box-binding protein 1;E3 SUMO-protein ligase PIAS1;E3 SUMO-protein transferase PIAS1;GBP;GU/RH-II;gu-binding protein;protein inhibitor of activated STAT protein 1;RNA helicase II-binding protein;zinc finger, MIZ-type containing 3;ZMIZ3. |
NCBI Gene Id | 8554 |
Protein Name | E3 SUMO-protein ligase PIAS1 |
Description of Target | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Sumoylates PML (at'Lys-65' and 'Lys-160') and PML-RAR and promotes their ubiquitin-mediated degradation. PIAS1-mediated sumoylation of PML promotes its interaction with CSNK2A1/CK2 which in turn promotes PML phosphorylation and degradation (By similarity). Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Plays a dynamic role in adipogenesis by promoting the SUMOylation and degradation of CEBPB (By similarity). |
Uniprot ID | O75925 |
Protein Accession # | NP_057250.1 |
Molecular Weight | 71 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PIAS1 Antibody (OAAF01956)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse".
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How long will it take to receive "PIAS1 Antibody (OAAF01956)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "PIAS1 Antibody (OAAF01956)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PIAS1 Antibody (OAAF01956)"?
This target may also be called "AR interacting protein;DDXBP1;DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1;DEAD/H box-binding protein 1;E3 SUMO-protein ligase PIAS1;E3 SUMO-protein transferase PIAS1;GBP;GU/RH-II;gu-binding protein;protein inhibitor of activated STAT protein 1;RNA helicase II-binding protein;zinc finger, MIZ-type containing 3;ZMIZ3." in publications.
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What is the shipping cost for "PIAS1 Antibody (OAAF01956)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PIAS1 Antibody (OAAF01956)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PIAS1 Antibody (OAAF01956)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "71 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PIAS1 Antibody (OAAF01956)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "PIAS1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "PIAS1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "PIAS1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "PIAS1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "PIAS1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "PIAS1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.