PIAS1 Antibody - N-terminal region (OAAB08696)

Catalog No: OAAB08696

Size:400ul

The PIAS1 polyclonal antibody (Cat. #OAAB08696) is used in Western blot to detect PIAS1 in HL-60 tissue lysate.

Western blot analysis of PIAS1 (arrow) using rabbit polyclonal PIAS1 Antibody (N-term) (RB00668). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIAS1 gene (Lane 2) (Origene Technologies).

There is BioGPS gene expression data showing that PIAS1 is expressed in HL60

PIAS1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: PIAS1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling
  Cellular Location: Nucleus speckle. Note=Interaction with CSRP2 may induce a partial redistribution along the cytoskeleton
  Tissue Location: Expressed in numerous tissues with highest level in testis
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PIAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-130 amino acids from the N-terminal region of human PIAS1.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  There is BioGPS gene expression data showing that PIAS1 is expressed in HL60

  PIAS1 is strongly supported by BioGPS gene expression data to be expressed in HEK293

• Application Info: WB: 1:1000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: PIAS1
• Alias Symbols: GBP, ZMIZ3, DDXBP1, GU/RH-II
• NCBI Gene Id: 8554
• Protein Name: E3 SUMO-protein ligase PIAS1
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O75925
• Protein Accession #: NP_057250.1
• Molecular Weight: 71836 Da