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PIAS1 Antibody - middle region (ARP39232_P050)

100 ul
$289.00
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Conjugation Options

ARP39232_P050-FITC Conjugated

ARP39232_P050-HRP Conjugated

ARP39232_P050-Biotin Conjugated

Gene Symbol:
PIAS1
Official Gene Full Name:
Protein inhibitor of activated STAT, 1
NCBI Gene Id:
8554
Protein Name:
E3 SUMO-protein ligase PIAS1
Swissprot Id:
O75925
Protein Accession #:
NP_057250
Nucleotide Accession #:
NM_016166
Alias Symbols:
DDXBP1, GBP, GU/RH-II, MGC141878, MGC141879, ZMIZ3
Replacement Item:
This antibody may replace item sc-14016 from Santa Cruz Biotechnology.
Description of Target:
PIAS1 is a member of the mammalian PIAS [protein inhibitor of activated STAT-1 (signal transducer and activator of transcription-1)] family. This member contains a putative zinc-binding motif and a highly acidic region. It inhibits STAT1-mediated gene act
Protein Size (# AA):
651
Molecular Weight:
72kDa
Host:
Rabbit
Clonality:
Polyclonal
Purification:
Affinity Purified
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express PIAS1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express PIAS1.
Immunogen:
The immunogen is a synthetic peptide directed towards the middle region of human PIAS1
Predicted Species Reactivity:
Cow, Dog, Horse, Human
Tested Species Reactivity:
Human
Predicted Homology Based on Immunogen Sequence:
Cow: 79%; Dog: 93%; Horse: 79%; Human: 100%
Complete computational species homology data:
Anti-PIAS1 (ARP39232_P050)
Peptide Sequence:
Synthetic peptide located within the following region: LPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISL
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
STAT1; TRIM63; TRIM55; CEP250; PRDM1; SUMO1; L3MBTL2; CBS; SHFM1; SUMO2; SUMO3; SOX2; CHUK; SPOP; YWHAZ; UBC; SKIL; SGTA; ATXN1; SMAD7; SMAD4; SMAD1; HTT; PRPF40A; PPP1CC; BARD1; PAXIP1; NR1H2; PPARGC1A; NR1H3; PPARGC1B; RAD54L2; ZMYND11; UBD; PLAG1; MYB;
Blocking Peptide:
For anti-PIAS1 (ARP39232_P050) antibody is Catalog # AAP39232 (Previous Catalog # AAPY00844)
Datasheets/Manuals:
Printable datasheet for anti-PIAS1 (ARP39232_P050) antibody
Sample Type Confirmation:

There is BioGPS gene expression data showing that PIAS1 is expressed in COLO205

Target Reference:
Helbig,K.J., (2008) Antiviral Res. 77 (3), 169-176

Product Reviews

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02/01/2017 16:23
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Product Review: PIAS1 antibody - middle region (ARP39232_P050) in Untreated and treated human U251 cell using Western blot
Product page for PIAS1 antibody - middle region (ARP39232_P050)

Researcher: Anonymous
Application: Western blotting
Species + Tissue/Cell type: Lane 1: Untreated human U251 lysate Lane 2: IL-1beta treated human U251 lysate
Primary antibody dilution: 1:500
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:500


Questionnaire:
How do Aviva's reagents play a role in your experimental goals? Regulation of the expression of PIAS1 in glial cells
How would you rate this antibody on a scale from 1-5 (5=best) and why? 5 - not work properly
Would you use this antibody in future experiments? Probably
Have you used another antibody which has worked in your application? No
Do you believe the information about the reagent on Aviva's website is correct? Yes
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? May be
How did you store the antibody after re-suspension?  -20C
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): Human; Glioblastoma cells
How many different experimental trials were conducted using the antibody sample? 2
How was this sample prepared? Cell cultures in 60 mm dishes were scraped into lysis buffer (PBS with a cocktail of protease inhibitors (Sigma) plus 1% Triton X-100), incubated for 30 min. at 4C with constant rotation, and centrifuged at 10,000 rpm for 10 min.
Primary antibody dilution and incubation time:  1:500
Secondary antibody used and dilution and incubation time:  1:500
Please include your detailed WB Procedure/Protocol here: Western blotting.
1. Cell cultures in 60 mm dishes were scraped into lysis buffer (PBS with a cocktail of protease inhibitors (Sigma) plus 1% Triton X-100), incubated for 30 min. at 4C with constant rotation, and centrifuged at 10,000 rpm for 10 min. The protein concentration was measured with Bio-Rad Protein assay (#500-0006).
2. Forty micrograms of protein was separated by electrophoresis in 10% sodium dodecyl sulfate-polyacrylamide gel and then transferred to polyvinylidene difluoride membrane (Bio-Rad#162-0177) at 60V for 3h in cold room (4C).
3. The blots were blocked in 5% nonfat milk (Bio-Rad #170-6404) prepared in PBS + 0.1% Tween-20 for 1 h at room temperature (RT) with constant shaking.
4.  Then the membranes were incubated with Primary antibodies o/n (~18h) at 4C with constant rotation.
5. After washing (shaking 3 times for 5 min each in PBS+0.1% Tween-20) the Secondary antibodies (horseradish peroxidase-conjugated anti-rabbit IgG (Thermo Scientific/Pierce#1858415)) were applied at dilution 1:500 - 1:1,000 for 1 h at RT with constant rotation.
6. After washing (3 times for 5 min each) the signals were detected with SuperSignal West Pico (or Femto)  Chemiluminescent Substrate (Thermo Scientific/Pierce #34077 or #34095).
7. All blots were reprobed for b-actin to control the protein loading. At first, the membranes were stripped with Restore PLUS Western Blot Stripping Buffer (Thermo Scientific#46430), then blocked in 5% milk (see step 3), and then incubated with anti-Actin Ab (1:500) o/n at 4C, washed, and incubated with  horseradish peroxidase-conjugated anti-rabbit IgG (1:500) followed washing and ECL development.
We used CL-XPosure Film (Thermo Scientific#34090).
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