PI4K2A Antibody - N-terminal region (OAAB17392)

Catalog No: OAAB17392

Size:400ul

Western blot analysis of anti-PI4K II Pab (Cat. # OAAB17392) in HL60 cell lysate. PI4K II (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Western blot analysis of anti-PI4K2A Antibody (N-term D2) in mouse brain tissue lysates (35ug/lane). PI4KII(arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Negative control of hela cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence. Immunofluorescence analysis of PI4K2A (PI4K II) Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: PI4K2A (PI4K II) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Together with PI4K2B and the type III PI4Ks (PIK4CA and PIK4CB) it contributes to the overall PI4-kinase activity of the cell. The phosphorylation of phosphatidylinositol (PI) to PI4P is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3). Contributes to the production of InsP3 in stimulated cells (By similarity)
  Cellular Location: Cytoplasm. Membrane (By similarity). Membrane raft. Cell projection, dendrite (By similarity). Cell junction, synapse, presynaptic cell membrane (By similarity). Cell junction, synapse, synaptosome (By similarity). Mitochondrion (By similarity). Endosome (By similarity). Cytoplasmic vesicle (By similarity). Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron project
  Tissue Location: Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:8000
  WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50

Target Info

• Gene Symbol: PI4K2A
• Alias Symbols: PI4KII, PIK42A
• NCBI Gene Id: 55361
• Protein Name: Phosphatidylinositol 4-kinase type 2-alpha
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9BTU6
• Protein Accession #: NP_060895.1
• Molecular Weight: 54022 Da