PI3KC3 Antibody (Phospho-S34) (OAAB14061)

Catalog No: OAAB14061

Size:400ul

Western blot analysis of PI3KC3 (S34) (Cat. #OAAB14061) in Hela cell line lysates (35ug/lane). PI3KC3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PI3KC3 Antibody (S34), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Fluorescent image of U251 cells stained with PI3KC3 (S34) antibody. U251 cells were treated with Chloroquine (50 ?M, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with OAAB14061 PI3KC3 (S34) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ?g/ml, 5 min). PI3KC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Pig
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IF, WB, IHC
• Additional Information: Precautions: PI3KC3 Antibody (S34) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes Required for the abcission step in cytokinesis. Required for transport from early to late endosomes
  Cellular Location: Midbody. Late endosome.
  Tissue Location: Ubiquitously expressed, with a highest expression in skeletal muscle
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-39 amino acids from human PI3KC3.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Pig; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: IF: 1:200
  WB: 1:1000
  IHC-P: 1:10~50
  IHC-P: 1:100

Target Info

• Gene Symbol: PIK3C3
• Alias Symbols: VPS34, Vps34, hVps34
• NCBI Gene Id: 5289
• Protein Name: Phosphatidylinositol 3-kinase catalytic subunit type 3
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q8NEB9
• Protein Accession #: NP_002638.2
• Molecular Weight: 101549 Da