PI3Kb Protein (OPPA01189)

Catalog No: OPPA01189 (Formerly GWB-5C5AB1)

Size:1UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: 0.5 mg/ml solution in 10mM Hepes, pH 7.5, 100mM NaCl, 2.5mM MgCl2, and 50% glycerol. Physical appearance: Sterile filtered liquid formualtion.
• Host: Sf9 Insect Cells
  Biological Activity: ~ 3 nmol/mg/min using phosphatidylinositol as the substrate.
  Product Introduction: The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation ofPI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr,HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis.PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line.It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors.
  Product Description: Phosphoinositide 3-kinase beta Human Recombinant is a glycosilated protein having a molecular weight as follows: p85* chain 83.5 kDa, p110* chain 124.3 kDa.
• Reconstitution and Storage: PI3Kb although stable at 4C for 1 week, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 90.0% as determined by SDS Page.

Target Info

• Gene Symbol: PI3Kb
• Alias Symbols: Phosphoinositide 3-kinase beta p110/p85b, PI3K beta, PI3Kb, PI3Kb Human, Phosphoinositide 3-kinase beta p110beta/p85alpha Human Recombinant
• Description of Target: Recombinant Human Phosphoinositide 3-kinase beta P110B/p85a
• Protein Size (# AA): Recombinant