- Rabbit immunoglobulin
- WB, ELISA, IP, IHC
- Phosphotyrosine conjugated to KLH
- Predicted Species Reactivity:
- Multi species
- Reconstitution and Storage:
- Store at -20C. Shipping in Blue Ice or 4C.
- Printable datasheet for OASE00382
- 2 ug/mL (WB), 0.5 ug/mL (ELISA), 10 ug/mg (IP)
- Additional Information:
- Background Info: Recognizes proteins phosphorylated on tyrosine residues. Does not cross-react with phosphoserine or threonine.
- Scientific Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).
In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (5). Phosphotyrosine can be detected through specific antibodies, and are
helpful in facilitating the identification of tyrosine kinase substrates (6).
- Target Reference:
- 1. Goto, H. et al. (2005). Nature Cell Biology 8: 180-187. 2. Blume-Jensen, P. and Hunter, T. (2001).
Nature 411: 355-365.
3. Downward, J. (2001). Nature 411: 759-762.
4. Pawson, T. and Saxton, T.M. (1999). Cell 97:
5. Frackelton, A.R. Jr., Ross, A.H., and Eisen,
H.N. (1983). Mol Cell Biol. 3: 1343-1352.
6. Ross, A.H., Baltimore, D., and Eisen, H.N.
(1981). Nature 294: 654-656.
7. Ostrovsky, PC. (1995). Genes Dev. 9(16):
- Storage Buffer: