Phosphotyrosine Antibody : HRP (OASE00112-HRP)

Catalog No: OASE00112-HRP

Size:100ug

Product Info

• Predicted Species Reactivity: Not species specific
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Monoclonal
• Clone: G104
• Isotype: IgG1
• Host: Mouse
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB, IP, IF, IHC
• Additional Information: Background Info: Reacts with phosphotyrosine, and detects the presence of phosphotyrosine in both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphothreonine.
  Scientific Background: Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (3).
  Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (4-6).
  In particular, the phosphorylation of tyrosine is considered one of the key steps in signal transduction and regulation of enzymatic activity (7). Phosphotyrosine can be detected through specific antibodies, and are helpful in facilitating the identification of tyrosine kinase substrates (8).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of phosphorylated tyrosine residues in 10ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin with 1-ethyl-3-(3'-dimentrylaminopropyl) carbodiimide.
• Purification: Protein G Purified
• Concentration: 0.6-0.7 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Garton A.J., Flint A.J., and Tonks N.K. (1996) Mol and Cell Bio 16(11): 6408-6418.
  2. Garton A.J., and Tonks N.K. (1999) J Bio Chem. 274(6):
  3811-3818.
  3. Goto H. et al. (2005) Nature Cell Biology 8: 180-187.
  4. Blume-Jensen P. and Hunter T. (2001) Nature 411:355-
  365.
  5. Downward J. (2001) Nature 411: 759-762.
  6. Pawson T. and Saxton T.M. (1999) Cell 97: 675-678.
  7. Frackelton A.R. Jr., Ross A.H., and Eisen H.N. (1983) Mol
  Cell Biol. 3: 1343-1352.
  8. Ross A.H., Baltimore D., and Eisen H.N. (1981) Nature
  294: 654-656.
  9. Tiganis T., Kemp B.E., and Tonks N.K. (1999) J. Bio Chem.
  274(39): 27768-27775.