Phosphothreonine Antibody (OASE00085)
|Predicted Species Reactivity||Not species specific|
|Product Format||Ascites, 0.02% sodium Azide|
|Application||WB, ICC, IF|
|Reconstitution and Storage||Store at -20C/1 year. Avoid freeze/thaw cycle.
Shipping in Blue Ice or 4C.
|Immunogen||Phosphothreonine conjugated to KLH.|
|Datasheets/Manuals||Printable datasheet for OASE00085|
|Specificity||Reacts with phosphothreonine, and detects the presence of phosphothreonine in proteins of both un-stimulated and stimulated cell lysates. Does not cross-react with phosphoserine or phosphotyrosine.|
|Application Info||WB (1:100)
optimal dilutions for assays should be determined by the user.
|Target Reference||1. Goto H. et al. (2005) Nature Cell Biology 8: 180-187. 2. Blume-Jensen P. and Hunter T. (2001) Nature 411: 355-365.
3. Downward J. (2001) Nature 411: 759-762.
4. Pawson T. and Saxton, T.M. (1999) Cell 97: 675-678.
|Description of Target||Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein's activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time (1). Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders (2-4).|
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
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