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Catalog No: OKEH02601 (Formerly GWB-GR579F)
Size:96T
Price: $625.00
SKU
OKEH02601
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02601
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Phosphatidylserine ELISA Kit (OKEH02601) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Phosphatidylserine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Phosphatidylserine and incubated. The Phosphatidylserine found in the sample or standards competes with the biotinylated Phosphatidylserine for limited binding sites on the immobilized anti-Phosphatidylserine antibody. Excess unbound biotinylated Phosphatidylserine and sample or standard Phosphatidylserine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Phosphatidylserine captured in the well and inversely proportional to the amount of Phosphatidylserine which was contained in the sample or standard.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 91%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Phosphatidylserine Microplate96 Wells (12 x 8 Well strips)
Phosphatidylserine Lyophilized Standard2 x 20 nmol
Sample Diluent1 x 20 mL
100X Phosphatidylserine-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: PS
Target Name: Phosphatidylserine
::Pubchem: 6323481
::Chemical formula: C13H24NO10P
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity0.26 ng/mL
SpecificityNatural and recombinant General Phosphatidylserine
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Description
Alias SymbolsPS, Phosphatidylserine, PS
Protein NamePhosphatidylserine
Description of TargetPhosphatidylserine (abbreviated Ptd-L-Ser or PS) is a phospholipid component, usually kept on the inner-leaflet (the cytosolic side) of cell membranes by an enzyme called flippase. When a cell undergoes apoptosis, phosphatidylserine is no longer restricted to the cytosolic part of the membrane, but becomes exposed on the surface of the cell.
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