Catalog No: OKCA00507
Size:96 Wells
Price: $857.00
SKU
OKCA00507
Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.

Phosphatidylethanolamine Antibody (IgM) (Human) ELISA Kit (OKCA00507)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit PrincipleAviva Systems Biology Phosphatidylethanolamine Antibody (IgM) (Human) ELISA Kit (OKCA00507) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Phosphatidylethanolamine antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanIgM is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Phosphatidylethanolamine Antibody (IgM) captured in well.
ELISA Kit ReproducibilityThree different samples of known Phosphatidylethanolamine Antibody (IgM) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%
ELISA Kit Component
ComponentAmount
Phosphatidylethanolamine Antibody (IgM) Microplate96 Wells (12 x 8 Well strips)
Negative Control 1 x 1 mL
Positive Control 1 x 1 mL
Anti-Human IgM HRP-conjugate 1 x 12 mL
Sample Diluent 1 x 22 mL
30X Wash Buffer1 x 20 mL
Substrate A 1 x 5 mL
Substrate B 1 x 5 mL
Stop Solution 1 x 5 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum and Plasma
Assay InfoAssay Methodology: Qualitative Reverse Capture ELISA
Protocol Information
Protein NamePhosphatidylethanolamine Antibody (IgM)
Description of TargetPhosphatidylethanolamine Antibody (IgM)
Write Your Own Review
You're reviewing:Phosphatidylethanolamine Antibody (IgM) (Human) ELISA Kit (OKCA00507)
Your Rating