Phosphate Buffered Saline/Casein Block and Diluent - 5X Concentrate (GWB-43C323)

Catalog No: GWB-43C323

Product Info

• Application: ELISA
• Concentration: 5X
• Description: Phosphate Buffered Saline (PBS)/Casein Block and Diluent - 5X Concentrate consists of many phosphoserine groups that function to bind Ca ²⁺. The solution is ready for dilution to specific application requirements. GenWay blocking and diluent reagents contribute to the minimization of non-specific interactions of the secondary reagents with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. Phosphate Buffered Saline (PBS), pH 7.4, is a poor buffer above pH 7.5. Phosphate buffer should not be used in assays where competition for phosphate groups or complex formation with a metal ion is essential for the enzyme activation. Phosphate ions will inhibit carboxypeptidiase, carboxylase, urease, muscle diaminase, formase and phosphoglucomutase.