Catalog No: OPCA05091
Price: $0.00
SKU
OPCA05091
Availability: Domestic: within 4-6 weeks delivery | International: 4-6 weeks
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Datasheets/Manuals | Printable datasheet for OPCA05091 |
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Predicted Species Reactivity | Escherichia coli |
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Product Format | Liquid |
Additional Information | Species Specificity Detail: Escherichia coli (strain K12) |
Reconstitution and Storage | Briefly centrifuge lyophilized product prior to opening to bring the contents to the bottom. Please reconstitute protein to 0.1-1.0 mg/mL by adding deionized sterile water first, followed by addition of glycerol to a final concentration of 5-50%. Reconstituted product should be aliquoted for long-term storage at -20C/-80C. Our in house default final concentration of glycerol is 50% for reference. The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Formulation | Tris-base, 50% glycerol |
Purity | Greater than 90% as determined by SDS-PAGE. |
Protein Sequence | Full Length: MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHIPDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKPFHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGYLFELR |
Storage Buffer | Tris-base, 50% glycerol |
Source | Yeast |
Tag | N-terminal 6xHis-tagged and C-terminal Myc-tagged |
Reference | Escherichia coli purB gene: cloning, nucleotide sequence, and regulation by purR. He B., Smith J.M., Zalkin H. J. Bacteriol. 174:130-136(1992) |
Gene Symbol | PHOP |
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Alias Symbols | ECK1116 |
NCBI Gene Id | 945697 |
Protein Name | Transcriptional regulatory protein PhoP |
Description of Target | Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg2+ environments and the control of acid resistance genes. In low periplasmic Mg2+, PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg2+, PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG |
Uniprot ID | P23836 |
Protein Accession # | NP_415648 |
Nucleotide Accession # | NC_000913 |
Molecular Weight | 29 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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