PHB Antibody - C-terminal region (OABB01078)

Catalog No: OABB01078

Size:100UG

Anti-Prohibitin antibody, OABB01078, Western blotting
Lane 1: Rat Lung Tissue Lysate
Lane 2: Rat Skeletal Muscle Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: Rat Kidney Tissue Lysate
Lane 5: HELA Cell Lysate
Lane 6: MCF-7 Cell Lysate
Lane 7: PC-12 Cell Lysate
Lane 8: A549 Cell Lysate
Lane 9: SMMC Cell Lysate

Anti-Prohibitin antibody, OABB01078, IHC(P)
IHC(P): Human Lung Cancer Tissue

Anti-Prohibitin antibody, OABB01078, ICC
ICC: HELA Cell

Product Info

• Tested Species Reactivity: Human, Mouse, Rat
• Predicted Species Reactivity: Hamster|Human|Mouse|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: PHB(Prohibitin), also known as PHB1, is a protein that in humans is encoded by the PHB gene. White et al.(1991) mapped the PHB gene to chromosome 17 by analysis of human-mouse somatic cell hybrid cell lines using a genomic fragment of human prohibitin DNA isolated from a library using the rat prohibitin cDNA clone. By in situ hybridization, they localized the gene to 17q21. Sato et al.(1992) isolated the human homolog of the rat prohibitin gene and mapped it to 17q12-q21 by in situ hybridization. Proliferation of tumor cells depends on new blood vessel formation(angiogenesis) that accompanies malignant progression. Anticancer therapies using angiogenesis inhibitors or cytotoxic agents targeted to the vasculature of tumors have been evaluated in clinical trials. Although white fat is a nonmalignant tissue, it has the capability to quickly proliferate and expand. Furthermore, it is highly vascularized. Rupnick et al.(2002) showed that nonspecific angiogenesis inhibitors can prevent the development of obesity of mice.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
  Immunocytochemistry/Immunofluorescence : 2 ug/ml: Human: Mouse, Rat
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Prohibitin(PHB) detection. Tested with WB, IHC-P, ICC/IF in Human;Mouse;Rat.

Target Info

• Gene Symbol: PHB
• Gene Full Name: prohibitin
• Alias Symbols: epididymis luminal protein 215;epididymis secretory sperm binding protein Li 54e;HEL-215;HEL-S-54e;PHB1;prohibitin.
• NCBI Gene Id: 5245
• Protein Name: Prohibitin
• Description of Target: Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.
• Uniprot ID: P35232
• Molecular Weight: 29804 MW