Pgp ELISA Kit (Rat) (OKCD01598)

Catalog No: OKCD01598 (Formerly GWB-KBBTL9)

Size:96WELLS

Rat Pgp ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Permeability Glycoprotein (Pgp). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Permeability Glycoprotein (Pgp). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Permeability Glycoprotein (Pgp), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Permeability Glycoprotein (Pgp) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Permeability Glycoprotein (Pgp) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Permeability Glycoprotein (Pgp) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Pgp Microplate: 96 Wells (12 x 8 Well Strips)
  • Pgp Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Pgp Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Permeability Glycoprotein (Pgp). No significant cross-reactivity or interference between Permeability Glycoprotein (Pgp) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: PGP
• Gene Full Name: Phosphoglycolate phosphatase
• Alias Symbols: ATP-binding cassette sub-family B member 1;P-glycoprotein 1.
• NCBI Gene Id: 287115
• Protein Name: Multidrug resistance protein 1
• Description of Target: Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells.
• Uniprot ID: P43245
• Protein Accession #: NP_001162623.1
• Nucleotide Accession #: NM_001169152.1
• Protein Size (# AA): 321