Catalog No: OKCD00925 (Formerly GWB-KBBJO1)
Size:96WELLS
Price: $675.00
SKU
OKCD00925
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Datasheets/ManualsPrintable datasheet for PGLYRP1 ELISA Kit (Human) (OKCD00925)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)93-101%98-105%95-104%79-91%
EDTA plasma(n=5)97-104%96-104%84-98%88-97%
heparin plasma(n=5)99-105%96-103%78-101%80-89%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peptidoglycan Recognition Protein 1 (PGLYRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peptidoglycan Recognition Protein 1 (PGLYRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peptidoglycan Recognition Protein 1 (PGLYRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peptidoglycan Recognition Protein 1 (PGLYRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.78-50ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)88-10594
EDTA plasma(n=5)91-10196
heparin plasma(n=5)81-10293
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peptidoglycan Recognition Protein 1 (PGLYRP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peptidoglycan Recognition Protein 1 (PGLYRP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-PGLYRP1 Microplate96 Wells (12 x 8 Well Strips)
PGLYRP1 Lyophilized Standard2 x 20 ng
100X Biotinylated PGLYRP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, cell lysates and other biological fluids.
Sensitivity< 0.124 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Peptidoglycan Recognition Protein 1 (PGLYRP1). No significant cross-reactivity or interference between Peptidoglycan Recognition Protein 1 (PGLYRP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPGLYRP1
Gene Full Namepeptidoglycan recognition protein 1
Alias Symbolspeptidoglycan recognition protein 1;Peptidoglycan recognition protein short;PGLYRP;PGRP;PGRPS;PGRP-S;TAG7;TNF superfamily, member 3 (LTB)-like (peptidoglycan recognition protein);TNFSF3L.
NCBI Gene Id8993
Protein NamePeptidoglycan recognition protein 1
Description of TargetPattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity.
Uniprot IDO75594
Protein Accession #NP_005082
Nucleotide Accession #NM_005091.2
Protein Size (# AA)196
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