PGLYRP1 ELISA Kit (Human) (OKCA02130)

Catalog No: OKCA02130

Size:96 Wells

Human PGLYRP1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	90	85-94
  Serum (n=4)	1:2	97	92-101
  Serum (n=4)	1:4	103	99-107
  Serum (n=4)	1:8	84	80-88

• ELISA Kit Principle: Aviva Systems Biology PGLYRP1 ELISA Kit (Human) (OKCA02130) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PGLYRP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PGLYRP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PGLYRP1 captured in well.
• ELISA Kit Range: 15.6 pg/mL-1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	96	91-100
  EDTA plasma (n=4)	91	85-95

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-PGLYRP1 Microplate: 96 Wells (12 x 8 Well strips)
  • PGLYRP1 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated PGLYRP1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: PGLYRP1
• Alias Symbols: PGRP, TAG7, PGRPS, PGLYRP, PGRP-S, TNFSF3L
• NCBI Gene Id: 8993
• Protein Name: Peptidoglycan recognition protein 1
• Description of Target: Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity.
• Uniprot ID: O75594
• Protein Accession #: NP_005082
• Nucleotide Accession #: NM_005091