Pgf ELISA Kit (Mouse) (OKBB00833)

Catalog No: OKBB00833

Size:96 wells

Pgf ELISA Kit (Mouse) (OKBB00833) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse PLGF-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PLGF-2 has been precoated onto 96-well plates. Standards (Expression system for standard: sf21, Immunogen sequence: A24-P158) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PLGF-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse PLGF-2 amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	33	176	474	34	182	481
  Standard deviation	2.01	12.14	20.38	2.24	13.83	20.68
  CV(%)	6.1	6.9	4.3	6.6	7.6	4.3

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse Pgf Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse Pgf Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse Pgf Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	33	40	36	33	35	2.87	8.2%
  Sample 2	176	178	189	176	179	5.4	3%
  Sample 3	474	405	411	424	428	27.15	6.3%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
  O.D.	0.054	0.106	0.162	0.284	0.563	0.896	1.618	2.259

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <2 pg/ml
• Specificity: Natural and recombinant mouse PLGF-2
• Immunogen: Expression system for standard: sf21; Immunogen sequence: A24-P158
• Description: For Quantitative Detection of mouse PLGF-2 in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: PGF
• Gene Full Name: placental growth factor
• Alias Symbols: Placenta growth factor; PlGF; Pgf; Plgf
• NCBI Gene Id: 18654
• Protein Name: Placenta growth factor
• Description of Target: Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading maternal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.
• Uniprot ID: Q544A5
• Protein Accession #: NP_032853.1
• Nucleotide Accession #: NM_008827.3