Catalog No: OKEH03205
Size:96T
Price: $525.00
SKU
OKEH03205
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PFKM ELISA Kit (Rat) (OKEH03205) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pfkm has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pfkm is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pfkm captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Component
ComponentAmount
Anti-Pfkm Microplate96 Wells (12 x 8 Well strips)
Pfkm Lyophilized Standard2
100X Biotinylated Pfkm Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Rat 6-phosphofructokinase, muscle type
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPfkm
Gene Full Namephosphofructokinase, muscle
Alias Symbols6-phosphofructokinase type A;6-phosphofructokinase, muscle type;ATP-dependent 6-phosphofructokinase, muscle type;ATP-PFK;PFK-A;Pfk-M;phosphofructo-1-kinase isozyme A;phosphofructokinase 1;phosphofructokinase-A;phosphofructokinase-M;phosphohexokinase.
NCBI Gene Id65152
Protein NameP47858
Description of TargetATP-dependent 6-phosphofructokinase, muscle type
Uniprot IDhttps://www.uniprot.org/uniprot/P47858
Protein Accession #NP_113903.1
Nucleotide Accession #NM_031715.1
Protein Size (# AA)780
Molecular Weight86 kDa
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