PFKM ELISA Kit (Rat) (OKCD08474)

Catalog No: OKCD08474

Size:96WELLS

PFKM ELISA Kit (Rat) (OKCD08474) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphofructokinase, Muscle (PFKM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphofructokinase, Muscle (PFKM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphofructokinase, Muscle (PFKM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphofructokinase, Muscle (PFKM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphofructokinase, Muscle (PFKM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphofructokinase, Muscle (PFKM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • PFKM Microplate: 96 Wells (12 x 8 Well strips)
  • PFKM Lyophilized Standard: 2
  • 100X PFKM HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.112ng/mL

Target Info

• Gene Symbol: Pfkm
• Gene Full Name: phosphofructokinase, muscle
• Alias Symbols: 6-phosphofructokinase type A;6-phosphofructokinase, muscle type;ATP-dependent 6-phosphofructokinase, muscle type;ATP-PFK;PFK-A;Pfk-M;phosphofructo-1-kinase isozyme A;phosphofructokinase 1;phosphofructokinase-A;phosphofructokinase-M;phosphohexokinase.
• NCBI Gene Id: 65152
• Protein Name: ATP-dependent 6-phosphofructokinase, muscle type
• Description of Target: Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
• Uniprot ID: P47858
• Protein Accession #: NP_113903.1
• Nucleotide Accession #: NM_031715.1