Pentosidine ELISA Kit (OKEH02528)

Catalog No: OKEH02528

Size:96T

Pentosidine ELISA Kit (OKEH02528) standard curve.

Product Info

• Predicted Species Reactivity: All Species
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Pentosidine ELISA Kit (OKEH02528) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pentosidine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pentosidine and incubated. The Pentosidine found in the sample or standards competes with the biotinylated Pentosidine for limited binding sites on the immobilized anti-Pentosidine antibody. Excess unbound biotinylated Pentosidine and sample or standard Pentosidine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pentosidine captured in the well and inversely proportional to the amount of Pentosidine which was contained in the sample or standard.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 88%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.4% (n = 20)
  Mean Inter-assay CV%: < 8.1% (n = 20)
• ELISA Kit Component:
  • Anti-Pentosidine Microplate: 96 Wells (12 x 8 Well strips)
  • Pentosidine Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X Pentosidine-Biotin Complex: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Target Abbr: Pentosidine
  Target Name: Pentosidine
  Pubchem: 119593
  Chemical formula: C17H26N6O4
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
• Sensitivity: 0.51 ng/mL
• Specificity: Natural and recombinant General Pentosidine
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Alias Symbols: Pentosidine, Pentosidine, Pentosidine
• Protein Name: Pentosidine
• Description of Target: Pentosidine is a biomarker for advanced glycation endproducts, or AGEs. It is a well characterized and easily detected member of this large class of compounds.