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PENK ELISA Kit (Rat) (OKEH03078)

96 Wells
$529.00
In Stock
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Gene Symbol:
PENK
Official Gene Full Name:
proenkephalin
NCBI Gene Id:
29237
Alias Symbols:
Enk, MGC93514, Penk1, Penk2, Penk-rs, Proenkephalin-A
Description of Target:
Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(239-260) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
Protein Name:
Proenkephalin-A
Swissprot Id:
P04094
Protein Accession #:
NP_058835.1
Nucleotide Accession #:
NM_017139.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.078 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology PENK ELISA Kit (Rat) (OKEH03078) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Penk has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Penk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Penk captured in well.
Kit Component:
ComponentAmount
Anti-Penk Microplate96 Wells (12 x 8 Well strips)
Penk Lyophilized Standard2
100X Biotinylated Penk Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Penk.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Penk.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat Proenkephalin-A. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
31 kDa
Protein Size (# AA):
269

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