- Official Gene Full Name:
- NCBI Gene Id:
- Alias Symbols:
- Enk, MGC93514, Penk1, Penk2, Penk-rs, Proenkephalin-A
- Description of Target:
- Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(239-260) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
- Protein Name:
- Predicted Species Reactivity:
- Sample Type:
- Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
- 0.078 ng/mL
- Kit Range:
- 0.156 - 10 ng/mL
- Kit Duration:
- ~ 3 Hours
- Kit Principle:
- Aviva Systems Biology PENK ELISA Kit (Rat) (OKEH03078) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Penk has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Penk is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Penk captured in well.
- Kit Component:
|Anti-Penk Microplate||96 Wells (12 x 8 Well strips)|
|Penk Lyophilized Standard||2|
|100X Biotinylated Penk Detector Antibody||1 x 120 uL|
|100X Avidin-HRP Conjugate||1 x 120 uL|
|Sample Diluent||1 x 20 mL|
|Detector Antibody Diluent||1 x 12 mL|
|Conjugate Diluent||1 x 12 mL|
|25X Wash Buffer||1 x 30 mL|
|TMB Substrate||1 x 10 mL|
|Stop Solution||1 x 10 mL|
- Kit Detection Method:
- Colorimetric, OD450 nm
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express Penk.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express Penk.
- Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
- Reconstitution and Storage:
- Store as indicated in product manual.
- This assay recognizes recombinant and natural rat Proenkephalin-A. No significant cross-reactivity or interference was observed.
- Assay Info:
- Assay Methodology: Quantitative Sandwich Immunoassay