Catalog No: OKCD08471
Size:96WELLS
Price: $800.00
SKU
OKCD08471
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Datasheets/ManualsPrintable datasheet for PENK ELISA Kit (Human) (OKCD08471)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proenkephalin (PENK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Proenkephalin (PENK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proenkephalin (PENK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Proenkephalin (PENK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Proenkephalin (PENK) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Proenkephalin (PENK) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PENK Microplate96 Wells (12 x 8 Well strips)
PENK Lyophilized Standard2
100X PENK HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cerebrospinal fluid and other biological fluids
Sensitivity< 0.057ng/mL
Gene SymbolPENK
Gene Full Nameproenkephalin
Alias Symbolsenkephalin A;PE;PENK-A;peptide F;preproenkephalin;proenkephalin-A.
NCBI Gene Id5179
Protein NameProenkephalin-A
Description of TargetMet- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
Uniprot IDP01210
Protein Accession #NP_001129162.1
Nucleotide Accession #NM_001135690.2
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