Size:96 Wells
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PECAM1 ELISA Kit (Rat) (OKEH03098)

Catalog#: OKEH03098
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species ReactivityRat
Kit Detection MethodColorimetric, OD450 nm
Kit Duration~ 3 Hours
Kit PrincipleAviva Systems Biology PECAM1 ELISA Kit (Rat) (OKEH03098) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pecam1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pecam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pecam1 captured in well.
Kit Range0.156 - 10ng/mL
Kit Component
Anti-Pecam1 Microplate96 Wells (12 x 8 Well strips)
Pecam1 Lyophilized Standard2
100X Biotinylated Pecam1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.086 ng/mL
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
SpecificityNatural and recombinant Rat Platelet endothelial cell adhesion molecule
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPECAM1
Official Gene Full Nameplatelet and endothelial cell adhesion molecule 1
Alias SymbolsCD31, MGC124998, Pecam, PECAM-1, Platelet endothelial cell adhesion molecule
NCBI Gene Id29583
Protein NamePlatelet endothelial cell adhesion molecule
Description of TargetCell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation. Induces susceptibility to atherosclerosis.
Swissprot IdQ3SWT0
Protein Accession #NP_113779.1
Nucleotide Accession #NM_031591.1
Protein Size (# AA)678
Molecular Weight76 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Pecam1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Pecam1.
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