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PECAM1 ELISA Kit (Rat) (OKEH03098)

96 Wells
$529.00
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Gene Symbol:
PECAM1
Official Gene Full Name:
platelet and endothelial cell adhesion molecule 1
NCBI Gene Id:
29583
Alias Symbols:
CD31, MGC124998, Pecam, PECAM-1, Platelet endothelial cell adhesion molecule
Description of Target:
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Modulates BDKRB2 activation. Induces susceptibility to atherosclerosis.
Protein Name:
Platelet endothelial cell adhesion molecule
Swissprot Id:
Q3SWT0
Protein Accession #:
NP_113779.1
Nucleotide Accession #:
NM_031591.1
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
0.078 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology PECAM1 ELISA Kit (Rat) (OKEH03098) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pecam1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Pecam1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pecam1 captured in well.
Kit Component:
ComponentAmount
Anti-Pecam1 Microplate96 Wells (12 x 8 Well strips)
Pecam1 Lyophilized Standard2
100X Biotinylated Pecam1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Pecam1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Pecam1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat PECAM-1. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
76 kDa
Protein Size (# AA):
678

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