PECAM1 Antibody (OAAF00819)

Catalog No: OAAF00819 (Formerly GWB-ASC233)

Size:100 ug

Western blot analysis of extracts from Jurkat cells, using PECAM-1 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PECAM-1 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using PECAM-1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human PECAM-1.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
• Specificity: PECAM-1 antibody detects endogenous levels of total PECAM-1 protein.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB 1:500~1000
  IHC 1:50~100
  IF 1:100~500
  ELISA 1:20000
  
• Reference: Tieming Zhao, J. Cell Biol., Jan 2001; 152: 65.
  SM Watt, Blood, Nov 1993; 82: 2649 - 2663.
  NETA ILAN, FASEB J, Feb 2001; 15: 362.

Target Info

• Gene Symbol: PECAM1
• Gene Full Name: platelet and endothelial cell adhesion molecule 1
• Alias Symbols: CD31;CD31 antigen;CD31/EndoCAM;endoCAM;GPIIA';PECA1;PECAM-1;platelet endothelial cell adhesion molecule;platelet endothelial cell adhesion molecule-1.
• NCBI Gene Id: 5175
• Protein Name: Platelet endothelial cell adhesion molecule
• Description of Target: Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis (By similarity).
• Uniprot ID: P16284
• Protein Accession #: NP_000433.4
• Molecular Weight: 82 kDa