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Catalog No: OKCD08955
Size:96WELLS
Price: $800.00
SKU
OKCD08955
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Datasheets/ManualsPrintable datasheet for PEBP1 ELISA Kit (Human) (OKCD08955)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phosphatidylethanolamine Binding Protein 1 (PEBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatidylethanolamine Binding Protein 1 (PEBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Phosphatidylethanolamine Binding Protein 1 (PEBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phosphatidylethanolamine Binding Protein 1 (PEBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phosphatidylethanolamine Binding Protein 1 (PEBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
PEBP1 Microplate96 Wells (12 x 8 Well strips)
PEBP1 Lyophilized Standard2
100X PEBP1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.057ng/mL
Gene SymbolPEBP1
Gene Full Namephosphatidylethanolamine binding protein 1
Alias Symbolsepididymis luminal protein 210;epididymis secretory protein Li 34;epididymis secretory protein Li 96;HCNP;HCNPpp;HEL-210;HEL-S-34;HEL-S-96;hippocampal cholinergic neurostimulating peptide;neuropolypeptide h3;PBP;PEBP;PEBP-1;phosphatidylethanolamine-binding protein 1;prostatic binding protein;Prostatic-binding protein;raf kinase inhibitor protein;Raf kinase inhibitory protein;RKIP.
NCBI Gene Id5037
Protein NamePhosphatidylethanolamine-binding protein 1
Description of TargetBinds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.
Uniprot IDP30086
Protein Accession #NP_002558.1
Nucleotide Accession #NM_002567.3
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