PDXP ELISA Kit (Mouse) (OKEH03398)

Catalog No: OKEH03398

Size:96T

Mouse PDXP ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology PDXP ELISA Kit (Mouse) (OKEH03398) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pdxp has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Pdxp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pdxp captured in well.
• ELISA Kit Range: 78-5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.6% (n = 20)
  Mean Inter-assay CV%: <= 8.3% (n = 20)
• ELISA Kit Component:
  • Anti-Pdxp Microplate: 96 Wells (12 x 8 Well strips)
  • Pdxp Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Pdxp Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 39.4 pg/mL
• Specificity: Natural and recombinant Mouse Pyridoxal phosphate phosphatase
• Publications:

  PLPP/CIN-mediated NEDD4-2 S448 dephosphorylation regulates neuronal excitability via GluA1 ubiquitination. Cell Death Dis. 10, 545 (2019). 31320629

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA
• Description:
  View product citations for PDXP ELISA Kit (Mouse) (OKEH03398) on CiteAb

Target Info

• Gene Symbol: Pdxp
• Gene Full Name: pyridoxal (pyridoxine, vitamin B6) phosphatase
• Alias Symbols: 1600027H05Rik;AB041662;Chr;chronophilin;chronophin;PLP phosphatase;PLPP;PLP-phosophatase;pyridoxal 5'-phosphate phosphatase;pyridoxal phosphate phosphatase.
• NCBI Gene Id: 57028
• Protein Name: P60487
• Description of Target: Pyridoxal phosphate phosphatase
• Uniprot ID: https://www.uniprot.org/uniprot/P60487
• Protein Accession #: NP_064667.2
• Nucleotide Accession #: NM_020271.3
• Protein Size (# AA): 292
• Molecular Weight: 32 kDa