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Catalog No: OKEH01229
Size:96T
Price: $600.00
SKU
OKEH01229
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PDXP ELISA Kit (Human) (OKEH01229) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDXP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDXP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDXP captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.0% (n = 20)
Mean Inter-assay CV%: < 7.8% (n = 20)
ELISA Kit Component
ComponentAmount
PDXP Microplate96 Wells (12 x 8 Well strips)
PDXP Lyophilized Standard2
100X Biotinylated PDXP Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.176 ng/mL
SpecificityNatural and recombinant Human Pyridoxal phosphate phosphatase
Publications

PLPP/CIN-mediated NEDD4-2 S448 dephosphorylation regulates neuronal excitability via GluA1 ubiquitination. Cell Death Dis. 10, 545 (2019). 31320629

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolPDXP
Gene Full Namepyridoxal phosphatase
Alias Symbolschronophin;CIN;dJ37E16.5;PLP;PLP phosphatase;pyridoxal (pyridoxine, vitamin B6) phosphatase;pyridoxal phosphate phosphatase;testicular secretory protein Li 36.
NCBI Gene Id57026
Protein NameQ96GD0
Description of TargetPyridoxal phosphate phosphatase
Uniprot IDhttps://www.uniprot.org/uniprot/Q96GD0
Protein Accession #NP_064711.1
Nucleotide Accession #NM_020315.4
Protein Size (# AA)296
Molecular Weight32 kDa
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