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PDXK ELISA Kit (Human) (OKEH01237)

Catalog#: OKEH01237
Domestic: within 1 week delivery | International: 1 week
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Duration ~ 3 Hours
Kit Principle Aviva Systems Biology PDXK ELISA Kit (Human) (OKEH01237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDXK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDXK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDXK captured in well.
Kit Range 0.312 - 20ng/mL
Kit Recovery Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116%
Kit Reproducibility Mean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 11.4% (n = 20)
Kit Component
PDXK Microplate96 Wells (12 x 8 Well strips)
PDXK Lyophilized Standard2
100X Biotinylated PDXK Detector Antibody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage Store as indicated in product manual.
Sample Type Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity 0.1 ng/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity Natural and recombinant Human Pyridoxal kinase
Assay Info Assay Methodology: Quantitative Sandwich ELISA
Gene Symbol PDXK
Official Gene Full Name pyridoxal (pyridoxine, vitamin B6) kinase
Alias Symbols C21orf124, C21orf97, DKFZp566A071, FLJ21324, FLJ31940, FLJ37311, MGC15873, MGC31754, MGC52346, PKH, PNK, PRED79, Pyridoxal kinase, Pyridoxine kinase
NCBI Gene Id 8566
Protein Name Pyridoxal kinase
Description of Target The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
Swissprot Id O00764
Protein Accession # NP_003672.1
Nucleotide Accession # NM_003681.4
Protein Size (# AA) 312
Molecular Weight 35 kDa
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express PDXK.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express PDXK.
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