Catalog No: OKEH01237
Size:96T
Price: $525.00
SKU
OKEH01237
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology PDXK ELISA Kit (Human) (OKEH01237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDXK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDXK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDXK captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 116%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 11.4% (n = 20)
ELISA Kit Component
ComponentAmount
PDXK Microplate96 Wells (12 x 8 Well strips)
PDXK Lyophilized Standard2
100X Biotinylated PDXK Detector Antibody1 x 120 Ă‚uL
100X Avidin-HRP Conjugate1 x 120 Ă‚uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Pyridoxal kinase
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolPDXK
Gene Full Namepyridoxal kinase
Alias SymbolsC21orf124;C21orf97;epididymis secretory sperm binding protein Li 1a;HEL-S-1a;HMSN6C;PKH;PNK;PRED79;pyridoxal (pyridoxine, vitamin B6) kinase;pyridoxal kinase;pyridoxamine kinase;pyridoxine kinase;vitamin B6 kinase.
NCBI Gene Id8566
Protein NameO00764
Description of TargetPyridoxal kinase
Uniprot IDhttps://www.uniprot.org/uniprot/O00764
Protein Accession #NP_003672.1
Nucleotide Accession #NM_003681.4
Protein Size (# AA)312
Molecular Weight35 kDa
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