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Datasheets/Manuals | Printable datasheet for PDPN Antibody (OACD06408) |
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Predicted Species Reactivity | Mouse |
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Product Format | Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Application | Immunocytochemistry|Immunohistochemistry|Immunoprecipitation|Western blot |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | Range: Gly23 - Val138 |
Purification | Affinity Purified; Protein A Purified |
Concentration | 0.5 mg/ml |
Preservative | 0.02% NaN3 |
Specificity | The antibody is a rabbit polyclonal antibody raised against PDPN. It has been selected for its ability to recognize PDPN in immunohistochemical staining and western blotting. |
Formulation | PBS, pH7.4, containing 0.02% NaN3, 50% glycerol |
Application Data | Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5,000 Optimal working dilutions must be determined by end user. |
Gene Symbol | Pdpn |
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Gene Full Name | podoplanin |
Alias Symbols | aggrus;E11;glycoprotein 38;Gp3;Gp38;OTS;OTS-8;PA2.;PA2.26 antigen;podoplanin;RAND;RANDAM-2;T1;T1a;T1alpha;T1-alpha;transmembrane glycoprotein E11. |
NCBI Gene Id | 14726 |
Protein Name | Podoplanin |
Description of Target | Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation (PubMed:14522983, PubMed:15231832, PubMed:20110424, PubMed:17616532). Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (By similarity). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (PubMed:25347465). Through binding with LGALS8 may participate to connection of the lymphatic endothelium to the surrounding extracellular matrix (By similarity). In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion (PubMed:10574709). Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (By similarity). Required for normal lung cell proliferation and alveolus formation at birth (PubMed:12654292). Does not function as a water channel or as a regulator of aquaporin-type water channels (By similarity). Does not have any effect on folic acid or amino acid transport (PubMed:12032185). |
Uniprot ID | Q62011 |
Protein Accession # | NP_001277751.1 |
Nucleotide Accession # | NM_001290822 |
Molecular Weight | 18 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "PDPN Antibody (OACD06408)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "PDPN Antibody (OACD06408)"?
This item is available "Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery".
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What buffer format is "PDPN Antibody (OACD06408)" provided in?
This item is provided in "Liquid. PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "PDPN Antibody (OACD06408)"?
This target may also be called "aggrus;E11;glycoprotein 38;Gp3;Gp38;OTS;OTS-8;PA2.;PA2.26 antigen;podoplanin;RAND;RANDAM-2;T1;T1a;T1alpha;T1-alpha;transmembrane glycoprotein E11." in publications.
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What is the shipping cost for "PDPN Antibody (OACD06408)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "PDPN Antibody (OACD06408)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "PDPN Antibody (OACD06408)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "18 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "PDPN Antibody (OACD06408)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Pdpn"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Pdpn"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Pdpn"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Pdpn"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Pdpn"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Pdpn"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.