PDPN Antibody (OABB00627)

Catalog No: OABB00627 (Formerly GWB-BBP550)

Size:100UG

Lane 1:Rat Brain Tissue Lysate
Lane 2:Rat Liver Tissue Lysate
Lane 3:SMMC Cell Lysate
Lane 4:HEPA Cell Lysate
Lane 5:293T Cell Lysate
Lane 6:HELA Cell Lysate

Product Info

• Tested Species Reactivity: Rat
• Predicted Species Reactivity: Mouse|Rat
• Product Format: Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: PDPN(Podoplanin), also called T1A, T1A2, GP36, OTS8 or AGGRUS, is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The PDPN gene is mapped to chromosome 1 by the International radiation Hybrid mapping consortium. The physiological function of PDPN may be related to its mucin-type character. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Immunohistochemical analysis of PDPN in placenta, kidney, lung, and nasal polyps showed expression at the apical plasma membrane of vascular endothelial cells and in alveolar epithelial cells. Overexpression of rat PDPN in human and rodent endothelial cells promoted formation of elongated cell extensions and significantly increased endothelial cell adhesion, migration, and tube formation. Inhibition of PDPN expression by small interfering RNAs decreased cell adhesion in cultured human dermal lymphatic endothelial cells.
  Background: PDPN(Podoplanin), also called T1A, T1A2, GP36, OTS8 or AGGRUS, is a protein that in humans is encoded by the PDPN gene. This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The PDPN gene is mapped to chromosome 1 by the International radiation Hybrid mapping consortium. The physiological function of PDPN may be related to its mucin-type character. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Immunohistochemical analysis of PDPN in placenta, kidney, lung, and nasal polyps showed expression at the apical plasma membrane of vascular endothelial cells and in alveolar epithelial cells. Overexpression of rat PDPN in human and rodent endothelial cells promoted formation of elongated cell extensions and significantly increased endothelial cell adhesion, migration, and tube formation. Inhibition of PDPN expression by small interfering RNAs decreased cell adhesion in cultured human dermal lymphatic endothelial cells.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: Polypeptide
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Mouse; Rat
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Rat: Mouse
• Reference: 1. Kato, Y., Fujita, N., Kunita, A., Sato, S., Kaneko, M., Osawa, M., Tsuruo, T. Molecular identification
  of Aggrus/T1-alpha as a platelet aggregation-inducing factor expressed in colorectal tumors. J. Biol.
  Chem. 278: 51599-51605, 2003.
  2. Ma, T., Yang, B., Matthay, M. A., Verkman, A. S. Evidence against a role of mouse, rat, and two
  cloned human T1-alpha isoforms as a water channel or a regulator of aquaporin-type water
  channels. Am. J. Resp. Cell Molec. Biol. 19: 143-149, 1998.
  3. Schacht, V., Ramirez, M. I., Hong, Y.-K., Hirakawa, S., Feng, D., Harvey, N., Williams, M., Dvorak,
  A. M., Dvorak, H. F., Oliver, G., Detmar, M. T1-alpha/podoplanin deficiency disrupts normal
  lymphatic vasculature formation and causes lymphedema. EMBO J. 22: 3546-3556, 2003
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Podoplanin(PDPN) detection. Tested with WB in Mouse;Rat.

Target Info

• Gene Symbol: Pdpn
• Gene Full Name: podoplanin
• Alias Symbols: E11;E11 antigen epitope;epithelial cell surface transmembrane protein antigen;glycoprotein 38;Gp38;OTS-8;podoplanin;pulmonary type I alveolar epithelial cell transmembrane differentiation marker;RTI140;RTI40;T1A;T1-alpha;type I cell 40 kDa protein.
• NCBI Gene Id: 54320
• Protein Name: Podoplanin
• Description of Target: Mediates effects on cell migration and adhesion through its different partners. During development plays a role in blood and lymphatic vessels separation by binding CLEC1B, triggering CLEC1B activation in platelets and leading to platelet activation and/or aggregation. Interaction with CD9, on the contrary, attenuates platelet aggregation and pulmonary metastasis induced by PDPN. Mediates effects on cell migration and adhesion through its different partners. Through MSN or EZR interaction promotes epithelial-mesenchymal transition (EMT) leading to ERZ phosphorylation and triggering RHOA activation leading to cell migration increase and invasiveness. Interaction with CD44 promotes directional cell migration in epithelial and tumor cells (By similarity). In lymph nodes (LNs), controls fibroblastic reticular cells (FRCs) adhesion to the extracellular matrix (ECM) and contraction of the actomyosin by maintaining ERM proteins (EZR; MSN and RDX) and MYL9 activation through association with unknown transmembrane proteins. Engagement of CLEC1B by PDPN promotes FRCs relaxation by blocking lateral membrane interactions leading to reduction of ERM proteins (EZR; MSN and RDX) and MYL9 activation (By similarity). Through binding with LGALS8 may participate to connection of the lymphatic endothelium to the surrounding extracellular matrix. In keratinocytes, induces changes in cell morphology showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Controls invadopodia stability and maturation leading to efficient degradation of the extracellular matrix (ECM) in tumor cells through modulation of RHOC activity in order to activate ROCK1/ROCK2 and LIMK1/LIMK2 and inactivation of CFL1 (By similarity). Required for normal lung cell proliferation and alveolus formation at birth (By similarity). Does not function as a water channel or as a regulator of aquaporin-type water channels (By similarity). Does not have any effect on folic acid or amino acid transport (By similarity).
• Uniprot ID: Q64294
• Protein Accession #: NP_001006625.1; NP_001006626.1; NP_006465.3; NP_938203.2; XP_006710358.1
• Nucleotide Accession #: NM_001006624.1; NM_001006625.1; NM_006474.4; NM_198389.2; XM_006710295.1
• Molecular Weight: 17579 MW