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Catalog No: OKCD01555 (Formerly GWB-KBBLY6)
Size:96WELLS
Price: $625.00
SKU
OKCD01555
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Datasheets/ManualsPrintable datasheet for Pdk4 ELISA Kit (Mouse) (OKCD01555)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)79-92%90-103%88-95%94-102%
EDTA Plasma (n=5)78-92%86-104%97-105%90-98%
Heparin Plasma (n=5)85-95%81-93%99-105%84-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Dehydrogenase Kinase 4 (PDK4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Dehydrogenase Kinase 4 (PDK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Dehydrogenase Kinase 4 (PDK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Pyruvate Dehydrogenase Kinase 4 (PDK4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-9995
EDTA Plasma (n=5)88-9693
Heparin Plasma (n=5)90-9794
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase 4 (PDK4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pyruvate Dehydrogenase Kinase 4 (PDK4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Pdk4 Microplate96 Wells (12 x 8 Well Strips)
Pdk4 Lyophilized Standard2 x 20 ng
100X Biotinylated Pdk4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.127 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4). No significant cross-reactivity or interference between Pyruvate Dehydrogenase Kinase Isozyme 4 (PDK4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolPdk4
Gene Full Namepyruvate dehydrogenase kinase, isoenzyme 4
Alias Symbols[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial;[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 4, mitochondrial;AV005916;pyruvate dehydrogenase kinase 4;Pyruvate dehydrogenase kinase isoform 4.
NCBI Gene Id27273
Protein Name[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial
Description of TargetKinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism in response to prolonged fasting and starvation. Plays an important role in maintaining normal blood glucose levels under starvation, and is involved in the insulin signaling cascade. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. In the fed state, mediates cellular responses to glucose levels and to a high-fat diet. Regulates both fatty acid oxidation and de novo fatty acid biosynthesis. Plays a role in the generation of reactive oxygen species. Protects detached epithelial cells against anoikis. Plays a role in cell proliferation via its role in regulating carbohydrate and fatty acid metabolism.
Uniprot IDO70571
Protein Accession #NP_038771.1
Nucleotide Accession #NM_013743.2
Protein Size (# AA)412
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